Preantral follicle culture as a novel in vitro assay in reproductive toxicology testing in mammalian oocytes
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چکیده
منابع مشابه
Aneuploidy in mouse metaphase II oocytes exposed in vivo and in vitro in preantral follicle culture to nocodazole.
Aneuploidy tests are important in evaluating genetic hazards especially when chemical exposures are suspected to affect the fidelity of chromosome segregation in oocytes and embryos. In the current study, a newly established method, mouse preantral follicle culture, was employed to grow oocytes in vitro within follicles. The sensitivity of in vitro grown follicle enclosed oocytes was compared w...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
development of mouse preantral follicle after in vitro culture in a medium containing melatonin
objective: improvements in cancer treatment have allowed more young women to survive. however, many cancer patients suffer from ovarian failure. cryopreservation is one of the solutions for fertility restoration in these patients. the cryopreservation of isolated follicles is a more attractive approach in the long term. many endocrine and paracrine factors can stimulate the granulosa cells of p...
متن کاملDevelopment of Mouse Preantral Follicle after In Vitro Culture in A Medium Containing Melatonin
OBJECTIVE Improvements in cancer treatment have allowed more young women to survive. However, many cancer patients suffer from ovarian failure. Cryopreservation is one of the solutions for fertility restoration in these patients. The cryopreservation of isolated follicles is a more attractive approach in the long term. Many endocrine and paracrine factors can stimulate the granulosa cells of pr...
متن کاملAn in vitro Comparative Study of Follicle Stimulating Hormone (FSH) and Activin A Effects on the Maturation of Preantral Follicle-Enclosed Oocytes from Immature Syrian Mice
Objectives: It was aimed to investigate the effects of different doses of follicle stimulating hormone (FSH) and activin A on the growth and maturation of preantral mouse follicles during the in vitro culture. Methods: Preantral follicles (90-100 µm in diameter) were harvested from 6-8 week-old Syrian mice and cultured in TCM199 culture medium for 6 days to see the effect of FSH and Activin A...
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ژورنال
عنوان ژورنال: Mutagenesis
سال: 2004
ISSN: 1464-3804
DOI: 10.1093/mutage/geg040